Fig. 4

TUG1 attenuates miR-524-5p mimic-mediated anti-tumor effects in HepG2. A The predictive binding of miR-524 and TUG1. Relative levels of miR-524 B in HepG2 cells with overexpression or silencing of miR-524-5p. C Binding of miR-524 and TUG1. D RIP experiments were performed in HepG2 cells with an AGO2 antibody, and the co-precipitated RNA was subjected to qPCR for TUG1. Effects of TUG1 overexpression and miR-524 mimic on (E, F) migration and invasion, (G) glucose uptake, (H) LDH activity, (I) lactate, and (J) ATP content, and (L, M) expression of MMP-2, MMP-9, HK2, and LDHA. The data are expressed as the mean ± SD (n = 3). ***P < 0.001 vs. blank or NC. ^###P < 0.001 compared with miR-524 mimic