Fig. 6

TUG1 negatively correlated with miR-524-5p and positively correlated with SIX1. qPCR assay of (A) TUG1, (B) miR-524-5p, and (C) SIX1 mRNA levels in adjacent non-tumor (n = 20), non-metastatic (n = 60), and metastatic tumor (n = 60) tissues. D–F Pearson correlation analysis among TUG1, miR-524-5p, and SIX1 mRNA levels in tumor tissues from 60 HCC patients with metastasis. G–I Survival probability of patients with HCC from hospital cohort, in which patients were split by the median value of TUG1, miR-524-5p, and SIX1 mRNA. The data are expressed as the mean ± SD (n = 20 or 60). **P < 0.01, ***P < 0.001 vs adjacent non-tumor. ^###P < 0.001 vs non-metastatic HCC tissues