Fig. 4

PADI2 induces ER stress-related proteins and inhibits mTOR and AKT phosphorylation in Tet-On-PADI2 Jurkat T cells. A Proteins extracted from Tet-On-Vector and Tet-On-PADI2 cells were detected using antibodies against PADI2, PERK, phosphorylated PERK, GRP78, IRE1α, phosphorylated eIF2α, and β-actin at the indicated time points after 50 μM Dox exposure. B Following 1 mM 4-PBA treatment, proteins extracted from Tet-On-Vector and Tet-On-PADI2 cells were detected using antibodies against PADI2, phosphorylated eIF2α, phosphorylated PERK, GRP78, p62, LC3B, and β-actin after 50 μM Dox exposure for 12 h. C Proteins extracted from Tet-On-Vector and Tet-On-PADI2 cells were detected using antibodies against AKT, phosphorylated AKT, mTOR, phosphorylated mTOR and β-actin at the indicated time points after 50 μM Dox exposure. The value indicates the ratio of protein signal to β-actin signal