Fig. 2

TRAP IL-1 inhibits CTX-induced gigantism while stimulating senescence morphology in SW48-CXR. SW48 and SW48 CXR cell lines (4 × 10⁴ cells per well) were seeded on 13 mm coverslip. After 24 h, SW48 cells were treated for 4 days with control medium (10% FBS), IL-1 (α and β, 10 ng/ml each), and CTX (50 μg/ml) alone and in combination with TRAP IL-1 (20 μg/ml). SW48 CXR cells were treated with control medium (10% FBS + CTX 50 μg/ml), and TRAP IL-1 (20 μg/ml). Subsequently, they were fixed and stained with DAPI (nuclei) and phalloidin (TRITCH) for 30 min. Finally, coverslips were mounted on slides and examined with a fluorescence microscope. ImageJ software was used to measure cell area of four different images taken for each sample. Percentage of cell number was calculated based on cell size and grouped in cells with area < or > 200 μm², represented by histograms for SW48 (a) and SW48 CXR (b). Statistical analysis was carried out using two-way ANOVA with Tukey’s and Sidak’s multiple comparisons test, respectively, ***p < 0.001, ****p < 0.001. Scale bar, 20 mm