Fig. 1

TMEM43 expression is upregulated and associated with poor survival in pancreatic cancer. A TMEM43 mRNA expression levels in different tumor samples and the corresponding normal samples (http://gepia.cancer-pku.cn/). B Western blotting and immunohistochemistry (IHC) assays showed the expression levels of TMEM43 in pancreatic cancer tissue samples and matched normal samples (n = 5). Scale bar (left) = 500 μm, scale bar (right) = 50 μm. C IHC showing the expression of TMEM43 in pancreatic cancer tissues and normal tissues in TMA cohort 1. Scale bar (left) = 200 μm, scale bar (right) = 50 μm. D Kaplan–Meier curve analysis showing the association of TMEM43 protein expression levels with patient survival using pancreatic cancer TMA cohort 2. E, F Kaplan–Meier curve analysis showing the correlation of TMEM43 mRNA levels with survival using the TCGA dataset. G, H Univariate and multivariate Cox regression analyses were performed in the TCGA pancreatic cancer dataset. *P < 0.05