Fig. 2

TMEM43 promotes pancreatic cancer proliferation, migration, and invasion in vitro. A The protein expression levels of TMEM43 were detected using western blotting in TMEM43-silenced cells and TMEM43-overexpressing MIAPaCa-2 cells. B, C Cell counting and colony formation assays showed the effects of silencing and overexpressing TMEM43 on MIAPaCa-2 cell proliferation. D The effects of silencing and overexpressing TMEM43 on pancreatic cancer cell cycle using flow cytometry assays. E, F The protein expression levels of TMEM43 were measured in TMEM43-knockdown SW1990, Capan-2 cells, and the corresponding control cells. G–J Cell counting and colony formation assays were performed in TMEM43-silenced SW1990, Capan-2 cells, and the corresponding control cells. K, L The cell cycle was analyzed in TMEM43-silenced SW1990, Capan-2 cells, and control cells with flow cytometry assays. M–O The migration and invasion abilities were detected in TMEM43-silenced MIAPaCa-2, SW1990, Capan-2 cells, TMEM43-overexpressing MIAPaCa-2 cells, and the corresponding control cells using transwell assays. Scale bar = 100 μm. Results are shown as the mean ± SD of three independent replicates. Scale bar = 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001