Fig. 2

RNF7 knockdown enhances apoptosis and reduces glycolysis in RCC cell lines. Caki-1 and Caki-2 cells were transduced with RNF7 shRNAs (shRNF7-1 and shRNF7-2) or control scramble shRNA (shNC). A Cell viability was measured by CCK-8 assay. B Cell apoptosis and C ECAR level were analyzed by flow cytometry. D Lactate release and E ATP content were measured by biochemical analyses. F The protein levels of HK2, GLUT1, cleaved PARP1, cleaved caspase-3, STAT3, and p-STAT3 in Caki-1 and Caki-2 cells were determined by Western blot. Experiments performed in triplicate; data expressed as mean ± SD (n = 3). ***P < 0.001 compared with the shNC group