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Fig. 6 | Cellular & Molecular Biology Letters

Fig. 6

From: HCMV-miR-US33-5p promotes apoptosis of aortic vascular smooth muscle cells by targeting EPAS1/SLC3A2 pathway

Fig. 6

Downregulating EPAS1 in HA-VSMCs promotes cell apoptosis. A–C EPAS1 was silenced in HA-VSMCs. qRT-PCR (A) and immunoblotting (C) analysis of EPAS1 in HA-VSMCs. qRT-PCR (B) and immunoblotting (C) analysis of SLC3A2 in HA-VSMCs. D Silencing SLC3A2 inhibited HA-VSMC proliferation. E, G Flow cytometry assay (E), caspase-3 activity assay (F), and Western blot analysis of cleaved caspase-3 (G) showed that silencing SLC3A2 promoted HA-VSMC apoptosis. H–K p-FAK (H), p-AKT (I), pY20CAS (J), and GTP-Rac (K) immunoblotting in HA-VSMCs. Western blot was performed three times; representative images are presented. Numbers above lanes indicate the relative protein level, normalized relative to control samples. ***P < 0.001, ****P < 0.0001 (n = 3, ANOVA analysis)

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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