Fig. 4

lncRNA SNHG12 bound to HuR to increase mRNA stability of PD-L1. A Localization of lncRNA SNHG12 in NSCLC cells was analyzed via the subcellular fractionation assay; B probability of lncRNA SNHG12 binding to HuR was predicted via the RNA–Protein Interaction Prediction (RPISeq) database; C the binding relationship between SNHG12 and HuR was analyzed using RIP assay; D probability of HuR binding to PD-L1 was predicted via the RPISeq database; E the binding relationship between PD-L1 and HuR was analyzed using RIP assay; F–I PD-L1 expression in NSCLC tissues and cells was measured via RT-qPCR and western blotting; J correlation between lncRNA SNHG12 and PD-L1 was analyzed via Pearson correlation analysis; A549 or H1299 cells were transfected by three strands of HuR siRNA (si-HuR), with si-NC as the negative control; K protein expression of HuR was detected via western blotting; L, M expression and half-life period of PD-L1 mRNA were determined via RT-qPCR. N = 65, cell experiments were performed three times, *P < 0.05, **P < 0.01. Data in A–E, G–I, and K–M are represented as mean ± standard deviation. Pairwise comparisons in F and G were analyzed using t-test; multigroup comparisons in C, E, H, and I were analyzed using one-way ANOVA, and in H, I, and K–M were analyzed using two-way ANOVA, followed by Tukey’s multiple comparison test