Fig. 8

PD-L1 overexpression attenuated the inhibition of silencing lncRNA SNHG12 on immune escape of NSCLC. A549 or H1299 cells were transfected with PD-L1 pcDNA3.1 (pc-PD-L1), with NC pcDNA3.1 (pc-NC) as the negative control. A, B PD-L1 expression in NSCLCs was measured via RT-qPCR and western blotting; A549 or H1299 cells in different treatment groups were co-cultured with isolated PBMCs. C PBMC proliferation was observed via Ki67 staining; D percentage of CD8⁺ T cells in PBMCs was detected via flow cytometry; E concentrations of TNF-α, IFN-γ, IL-10, and TGF-β were determined via ELISA; F cytotoxicity of PBMCs was detected via LDH cytotoxicity assay. Cell experiments were performed three times, *P < 0.05, **P < 0.01. Data are represented as mean ± standard deviation. Multigroup comparisons in A–F were analyzed using two-way ANOVA, followed by Tukey’s multiple comparison test