Fig. 1
From: Integrated analysis of tRNA-derived small RNAs in proliferative human aortic smooth muscle cells
Expression profile analysis and qRT-PCR detection of tsRNAs. A Scatter plot of DEtsRNAs between proliferative and quiescent HASMCs. TPM values (log2 scaled) of all tsRNAs; B Volcano plot of DEtsRNAs between proliferative and quiescent HASMCs; C Heatmap of DEtsRNAs between proliferative and quiescent HASMCs. Each row represents a tRNA, while each column represents a sample. P1, P2, and P3 represent three samples of proliferative HASMCs; Q1, Q2, and Q3 represent three samples of quiescent HASMCs; D Expression of four DEtsRNAs in proliferative and quiescent HASMCs was detected by qRT-PCR and normalized to U6. Data presented as mean ± SD of three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001 versus quiescent HASMCs. The p-value represents the possibility of a difference in tsRNA expression between proliferative and quiescent HASMCs; E AS-tDR-001370, AS-tDR-000067, AS-tDR-009512, and AS-tDR-000076 were abundant in the nucleus of HASMCs. U6 and GAPDH acted as positive controls in the nucleus and cytoplasm, respectively. Data shown as mean ± SD of three independent experiments