Raman microspectroscopy fingerprinting of organoid differentiation state

Table 1 Raman peak assignments reference table

Peak (cm⁻¹)	Peak assignment (specific wavenumber reference in parentheses)	References
569	Tryptophan/cytosine, guanine (573 cm⁻¹) inositol residue in phosphatidylinositol (576 cm⁻¹)	[38, 39]
621	C–C twisting mode of phenylalanine (621 cm⁻¹), tyrosine	[38]
676	Ring breathing modes in the DNA bases (674 cm⁻¹) Guanine ring breathing (679 cm⁻¹)	[38, 40]
745	DNA/tryptophan	[7]
920	C–C stretch of proline ring/glucose Collagen	[7, 15]
1003	Phenylalanine (1001–1004 cm⁻¹)	[40]
1124	C–C stretching mode of lipids/protein C–N stretch/glucose	[38]
1248	Amide III (collagen) (CH2 wag, C–N stretch)/pyrimidine bases (C, T)	[38]
1335	CH3CH2 twisting mode of protein, collagen (1335–1345 cm⁻¹) CH3CH2 deformation/wagging nucleic acid (1335–1339 cm⁻¹)	[7, 38]
1446	Phospholipid bands (1437–1442 cm⁻¹)	[39]
1654	Lipid (C=C stretch) (1652–1655 cm⁻¹) Amide I (1654–1659 cm⁻¹)	[38, 40, 41]
1926	CO stretching band	[42]

Select Raman peaks associated with quantification, tentative peak assignments, and references

ISSN: 1689-1392