Fig. 1

Expression of METTL3 was upregulated in hypertrophic myocardium induced by Ang-II. A NRCMs were treated with Ang-II to induce hypertrophy. Representative immunohistochemistry images of NRCMs stained with α-actinin in green. The nucleus is stained in blue. Scale bars, 10 μm. The cell surface area was evaluated. n = 3. B qRT-PCR detected the expression of hypertrophic biomarkers ANP and BNP in NRCMs stimulated with Ang-II. n = 3. C Western blot detected the expression of hypertrophic biomarkers ANP and BNP in NRCMs stimulated with Ang-II. n = 3. D qRT-PCR detected METTL3 expression in Ang-II-induced myocardial hypertrophy in vitro. n = 3. E Western blot detected the METTL3 expression in Ang-II-induced myocardial hypertrophy in vitro. n = 3. F Assay detected m6A modification of total RNA from Ang-II-induced NRCMs. n = 3. G Representative heart gross morphology. The HW:BW ratio was calculated by dividing heart weight by body weight. n = 5. H Western blot detected METTL3 expression in the myocardium of Ang-II treated mice. n = 5. *P < 0.05, **P < 0.01, ***P < 0.001