Fig. 6
From: WTAP-mediated N6-methyladenosine modification of NLRP3 mRNA in kidney injury of diabetic nephropathy
WTAP knockdown inhibits cell pyroptosis and pro-inflammatory factor release in db/db mice. A H&E and Masson’s trichrome staining of mouse kidney tissues (n = 6). Scale bar, 50 μm. B–D Serum creatinine, serum blood urea nitrogen, and urine protein levels (n = 6). (E) m6A levels measured by ELISA in primary tubular epithelial cells (n = 6). F RIP and RT-qPCR analysis of NLRP3 m6A levels in primary tubular epithelial cells (n = 6). G, H Expression of WTAP, NLRP3, pro-caspase-1, active caspase-1, GSDMD, and GSDMD-N in primary tubular epithelial cells (n = 3). (I, J) Serum levels of IL-18, IL-1β, TNF-α, and IL-6 (n = 6). K Pearson correlation scatter plots (n = 63). One-way analysis of variance was used to analyze the data among multiple groups, followed by Tukey’s post hoc test. ***P < 0.001 compared with db/m + shNC. #P < 0.05, ##P < 0.01, ###P < 0.001 compared with db/db + shNC