Fig. 4
From: SESN2 prevents the slow-to-fast myofiber shift in denervated atrophy via AMPK/PGC-1α pathway
Knockdown of SESN2 accelerated slow-to-fast fiber type shift in rotenone-treated C2C12 myotubes. A Western blot analysis confirming the effect of siSESN2. B Immunofluorescence analysis of slow and fast MHC. Scale bar, 50 μm. C Western blot analysis of proteins related to muscle fiber type. D, E Detection of relative ATP content and metabolic enzyme activities (LDH and SDH) in C2C12 myotubes. Data presented as mean ± SD. *P < 0.05 versus siNC group. #P < 0.05 versus rotenone treatment group. Rot rotenone