Fig. 1

Hsa-miR-27b is downregulated in HCC specimens, and low expression of hsa-miR-27b predicts poor prognosis. A Hsa-miR-27b expression in HCC samples and normal liver tissues was analysed using TCGA (HCC, n = 370; normal liver, n = 50; unpaired t-test) and GSE115016 (HCC, n = 12; normal liver, n = 12; unpaired t-test) datasets. B qRT-PCR analysis of the expression of hsa-miR-27b in HCC and adjacent non-tumourous tissues (ANTs) (Mann–Whitney test). Data were normalized to U6 and are shown as the fold change compared with patient 7. C ISH scoring of hsa-miR-27b in 71 pairs of HCC tissues (Mann–Whitney test). D Representative ISH images of hsa-miR-27b in paired HCC samples. Scale bar: overview images, 100 μm; magnified images, 200 μm. E Kaplan–Meier analysis of the correlation between hsa-miR-27b expression and overall survival in the cohort of patients with HCC (log-rank test). F Forest plot of the multivariate Cox proportional hazards model for overall survival. The bars correspond to 95% confidence intervals. *p < 0.05; **p < 0.01. Data are shown as mean ± SD. T, tumour; N, normal liver; ANTs, adjacent non-tumourous tissues; HCC, hepatocellular carcinoma; HR, hazard risk; CI, confidence interval