Fig. 5

The impacts of hsa-miR-27b on HCC cells were mediated by TAB3. A Representative images (left) and quantification (unpaired t-test) (right) of TAB3 IHC staining in 71 pairs of HCC tissues and ANTs. Scale bar: left, 100 μm; right, 200 μm. B Kaplan–Meier analysis of the relationship between TAB3 expression and overall survival of patients with HCC in the GEPIA database (log-rank test). C TAB3 mRNA and protein expression in HCC cell lines as determined by qRT-PCR (top) and western blots (bottom). Data were normalized to GAPDH and are shown as fold change compared with Hep3B cells for qRT-PCR (n = 3). GAPDH was used as a loading control for western blot analysis. D qRT-PCR (top) and western blot (bottom) analysis of TAB3 overexpression or knockdown efficacy in the indicated cells. Data were normalized to GAPDH and are shown as fold change relative to their respective control cells for qRT-PCR (n = 3, Mann–Whitney test). GAPDH was used as a loading control for western blot analysis. E CCK-8 assay of the indicated cells (n = 5, Kruskal–Wallis test). F Representative images of cell migration and invasion in the indicated groups (top). Scale bar, 200 μm. Quantification of cell migration and invasion (n = 3, Mann–Whitney test) (bottom). G CCK-8 assay for the indicated cell groups (n = 5, Kruskal–Wallis test). H Representative images and quantification of migrated and invaded cells in the indicated cell groups (n = 3, Mann–Whitney test). Scale bar, 200 μm. I Western blotting for EMT markers as indicated. GAPDH was used as a loading control. C–I were repeated three times. *p < 0.05. **p < 0.01. Data are shown as mean ± SD. ANTs, adjacent non-tumourous tissues; HCC, hepatocellular carcinoma; vec, vector; OE, overexpression; scr, scramble; sh, small hairpin RNA; ctrl, control; vec, vector; pc3.1-, pcDNA3.1-; KD, knockdown; sh, small hairpin RNA