Fig. 5

miR-576-3p mediates ox-LDL-induced HUVECs damage phenotype by targeting IFIT1. A Cluster heatmap exhibiting the abnormally expressed mRNAs from the microarray data. B Volcano map showing the abnormally expressed mRNAs. C Scatter plot presenting the abnormally expressed mRNAs from the microarray data. D Relative mRNA expression of IFIT1 was assessed in plaque tissues. *p < 0.05, **p < 0.001 versus the relative control group. E Relative mRNA expression of IFIT1 was assessed in ox-LDL-treated HUVECs. *p < 0.05, **p < 0.001 versus the relative control group. F Schematic illustration of the putative binding area for miR-576-3p associated with IFIT1. G Luciferase activity was revealed in cells coinfected with IFIT1-WT or IFIT1-MUT and miR-576-3p mimics and normal control (NC), respectively. H Relative IFIT1 mRNA levels were assessed in HUVECs via infection with miR-576-3p mimics and NC. I Relative IFIT1 protein expression was assessed in HUVECs via transfection with miR-576-3p mimics and NC. **p < 0.001 versus the NC group. J Relative IFIT1 mRNA levels were assessed in HUVECs via infection with miR-576-3p inhibitor and NC. K Relative IFIT1 protein expression was assessed in HUVECs via transfection with miR-576-3p inhibitor and NC. **p < 0.001 versus the NC group. L Viability of HUVECs infected with miR-576-3p mimics and overexpressed IFIT1 vector via CCK-8 assay. M Detection of migration potential of HUVECs infected with miR-576-3p mimics and overexpressed IFIT1 vector. N Detection of the vasculogenic capacity of HUVECs infected with miR-576-3p mimics and overexpressed IFIT1 vector. **p < 0.001 versus sham group, ^##p < 0.001 versus the relative control group