Fig. 4

The p62–Keap1–NRF2 pathway is involved in shCISD2-mediated ferroptosis. A Western blot analysis of the expression of proteins associated with the p62–Keap1–NRF2 pathway and ferroptosis in HT-1080 cells; GAPDH was used as loading control. B Cell viability assay in the indicated cells with or without Erastin treatment; cell viability in control cells was defined as 100%. C Representative images from flow cytometry for the detection of lipid peroxides by BODIPY staining in the indicated HT-1080 cells with or without Erastin treatment (7.5 µM). D Statistical graph of DCF fluorescence from microplate reader to detect the cellular ROS in the indicated HT-1080 cells with or without Erastin treatment (7.5 µM); ^★P < 0.05 and ^★★P < 0.01 between the indicated groups