Fig. 1

Relative SNHG16 expression in ESCC and IEN tissues and its clinical significance. A Hierarchical cluster heatmap of the differentially expressed lncRNAs in RNA-Seq analysis of four paired ESCC and adjacent tissues. Each row represents a gene, and each column represents a sample. Red represents upregulation of genes, and blue represents downregulation of genes. SNHG16 was one of the upregulated genes from next-generation RNA sequencing. TT, tumour tissue; TN, paired normal tissues. B SNHG16 expression in a larger independent cohort of 301 ESCC samples and 405 normal samples from the TCGA database (GSE53624, 53622). C SNHG16 expression was examined by qRT-PCR and normalized to GAPDH in 25 paired human ESCC tissues. D SNHG16 expression was examined by qRT-PCR and normalized to GAPDH in 30 pairs of ESD samples (*P < 0.05, **P < 0.01, ***P < 0.001)