Fig. 4

SNHG16 interacts with EIF4A3. SNHG16 expression in the cell nucleus or cytoplasm of ESCC cells, as detected by subcellular fractionation (A) and FISH (B) assays (scale bar, 100 μm). (C) Pull-down silver assay showed differential RBPs interacting with SNHG16 and antisense SNHG16. D LC–MS identified the differential RBPs binding to SNHG16 and anti-SNHG16. E The peptide sequence of EIF4A3, followed by LC–MS, specifically interacted with SNHG16. RIP F and Western blot G after pull down assays further validated the interaction between EIF4A3 and SNHG16. H Western blot analysis showed the expression of EIF4A3 after SNHG16 overexpression or downregulation. I qRT-PCR showed the expression of SNHG16 after EIF4A3 knockdown. *P < 0.05, **P < 0.01, ***P < 0.001