Fig. 5

SNHG16–EIF4A3 regulates RhoU and affects its mRNA stability. A A total of 204 transcripts were altered simultaneously in sh-SNHG16-treated cells and si-EIF4A3-treated cells (fold-change > 1.5, P < 0.05). B The hierarchical cluster heatmap of the 204 differentially expressed mRNAs in RNA-Seq analysis of sh-SNHG16-treated cells and si-EIF4A3-treated cells. Red represents upregulated transcripts, and blue represents downregulated transcripts. C qRT-PCR validated the changes in eight mRNAs involved in the regulation of sh-SNHG16 and si-EIF4A3. D Western blot analysis of RhoU after SNHG16 or EIF4A3 downregulation in ESCC cells. GAPDH was used as an internal control. E RIP showed that EIF4A3 interacted with RhoU and increased under SNHG16. F RNA stability assays were performed to measure the degradation rates of RhoU mRNA in KYSE30 and KYSE140 cells with SNHG16 or EIF4A3 knockdown. G RhoU mRNA stability in ESCC cells with LV-SNHG16 cells, KD-SNHG16 cells, and EIF4A3 knockdown in LV-SNHG16-treated cells and KD-SSNHG16 cells. *P < 0.05, **P < 0.01, ***P < 0.001