Fig. 1

Establishment of Ad-MSCs senescence model by 50 μM H₂O₂ treatment. A Percentage of Sa-β-gal-positive cells were significantly decreased under H₂O₂ treatment, Scale bar, 20 μm. n = 5 biological replicates. B H₂O₂ (50 μM) treatment inhibited the cell proliferation of Ad-MSCs. C The effect of H₂O₂ (50 μM) treatment on cell cycle phase by Flow cytometry analyses, n = 3 wells per cell type. D The expression of P21, P16 and P53 was down-regulated under H₂O₂ treatment