Fig. 7

Stathmin1 mutation at Ser16 or depletion of stathmin1 or TACC3 induces the formation of aberrant spindles. A Induction of multipolar and disorganized spindles in DLD1 cells transfected with a plasmid expressing a Ser16-mutated or wild-type stathmin1. After transfection for 48 h, the cells were fixed and stained with antibody to α-tubulin and DAPI. Bar, 10 pixels. B Quantification of the numbers of aberrant spindles in the DLD1 cells described in A. One hundred cells in each group were counted. C Induction of multipolar and disorganized spindles in HCT116 cells transfected with stathmin1, TACC3, or non-specific siRNA. After transfection for 48 h, cells were fixed and stained with antibody to α-tubulin antibody and DAPI. Bar, 10 pixels. D Quantification of the number of aberrant spindles in the HCT116 cells described in C. One hundred cells in each group were counted