Fig. 1

Cellular aberrancy in the mice brain under middle cerebral artery occlusion (MCAO). A Quality control, cell identity annotation, endothelial cell (EC) function and driving transcriptional factors identification, development path prediction, and downstream in vitro and in vivo validation were consecutively conducted. B UMAP visualization showing different cell types captured from combined sham (n = 3) and MCAO (n = 3) scRNA-seq data. C Dot plot showing the marker gene expression for each cell type. capEC capillary endothelial cell, vEC venous endothelial cell, aEC artery endothelial cell, SMC smooth muscle cell, Peri. pericyte, Peri.Fibro perivascular fibroblast, Mono. monocyte, Neutro. neutrophil, Inter.Micro interstitial microglia, Peri.Micro perivascular microglia, Astro astrocyte, Oligo. oligodendrocyte, Ependy. ependyma. D Bar plot showing cell proportion in each cell type by sample. E Bar plot showing cell proportion in each sample by cell type. F UMAP visualization showing EC subtypes in combined sham (n = 3) and MCAO (n = 3) data. G Dot plot showing the marker gene expression for each EC subtype. Art artery, Cap capillary. H Bar plot showing cell proportion in each sample by cell type