Fig. 2

Identification of healing-state endothelial cell as the major EC subtypes changed under MCAO condition. A UMAP visualization showing the pseudo-time trajectory of the cells (left) and the Scissor-positive (Scissor⁺) cells under sham (n = 3) and MCAO (n = 3) conditions (right). Scissor⁺ cells were more relevant to MCAO condition compared with NS cells. B Bar plot showing cell proportion in each EC subtype by Scissor results. The number of each cell subtype is labeled. C Violin plot comparing the cell proportions of Scissor⁺ healing ECs under sham (n = 3) and MCAO (n = 3) conditions. Student’s t-test was utilized. ***P < 0.001. D Heatmap plot showing the overall expression similarity of each EC subtype. E Ridge plot showing the UCell signature score of healing-state ECs. The UCell signatures of each EC subtype were defined using the top 30 marker genes filtered for FDR < 0.01. FDR false discovery rate. F Volcano plot showing the differentially expressed genes in healing ECs between MCAO (n = 3) and sham (n = 3) conditions. The filter criteria was set as FDR < 0.05 and |log₂FC|> 0.05. FC fold change. G Gene set enrichment analysis showing the putative functions of healing ECs under MCAO condition using the filtered differentially expressed genes