Fig. 4

ESCC exosomal delivery circ_0026611 promoted lymphangiogenesis by regulating PROX1. A–C HLECs were divided into three groups. The first group was treated with exosomes derived from KYSE30 cells transfected with vector. The second and third groups were treated with KYSE30-derived exosomes with circ_0026611 overexpression vector, but the third group was also transfected with sh-PROX1#1 at the same time. The cell migration, invasion, and tube formation in the three groups were assessed via functional assays. D–F HLECs were divided into three groups. The first group was treated with exosomes derived from EC109 cells transfected with sh-NC. The second and third groups were treated with EC109-derived exosomes with circ_0026611 inhibiting vector, but the third group was also transfected with pcDNA3.1-PROX1 at the same time. Transwell assay and tube formation assay were carried out to detect the cell migration, invasion, and tube formation in the three groups. **P < 0.01