Fig. 5

NAA10 interacting with circ_0026611 mediated PROX1 acetylation and promoted its ubiquitination and subsequent degradation. A The proteins that could interact with circ_0026611 were explored through RNA pulldown as well as mass spectrometry. B CoIP assay verified the interaction between NAA10 and PROX1. C The binding between GST-NAA10 and Flag-PROX1 was detected by GST-pulldown assay. D CoIP was used to detect whether endogenous PROX1 protein could be acetylated. E The effect of NAA10 on PROX1 acetylation was assessed by CoIP assay. F Western blot was used to determine the effect of NAA10 on PROX1 protein. G Western blot was used to assess the effect of NAM + TSA on PROX1 protein. H Protein levels of PROX1 in different treatments. I CoIP assay was used to verify the effect of NAA10 on PROX1 protein acetylation and ubiquitination