Fig. 5

Enhanced expression of Robo4 ameliorates the downregulation of TJs induced by IR. A–E Western blotting analysis showing the protein expression levels of claudin-5, occludin, PECAM-1, and ZO-1 in Robo4 overexpressed bEnd3 cells after irradiation. We used β-actin as a loading control. F and H Localization of claudin-5 and ZO-1 in microvascular EC samples by confocal microscopy following IR, respectively. The nuclear stain is DAPI. G Assessment of CD31 expression in the microvascular ECs 24 h post-irradiation using flow cytometry. Scale bars = 20 µm. For all experiments, n ≥ 3, and error bars represent std. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001