Table 1 Real-time quantitative PCR primer sequences
Genes | Primer sequences | |
|---|---|---|
Forward 5’ → 3’ | Reverse 5’ → 3’ | |
Robo4 | TTATGGCTCCCTCATCGCTG | GAGGCTGTCTGAGCTGGAAC |
ZO-1 | CAGCCGGTCACGATCTCCT | TCCGGAGACTGCCATTGC |
Occludin | CACACAGGACGTGCCTTCAC | GAGTATGCCATGGGACTGTCAA |
Claudin 5 | CTGCTGGTTCGCCAACATT | TGCGACACGGGCACAG |
PECAM-1 | CCAAAGCCAGTAGCATCATGGTC | GGATGGTGAAGTTGGCTACAGG |
Connexin 43 | TACCACGCCACCACTGGC | AATCTCCAGGTCATCAGG |
Connexin 40 | TTTGGCAAGTCACGGCAGGG | TTGTCACTGTGGTAGCCCTGAGG |
Connexin 37 | GGCTGGACCATGGAGCCGGT | TTTCGGCCACCCTGGGGAGC |
VE-cadherin | GAAGCCTCTGATTGGCACAGTG | TTTTGTGACTCGGAAGAACTGGC |
γ-Catenin | ACCAGCATCCTGCACAACCTCT | GGTGATGGCATAGAACAGGACC |
β-Catenin | CACAAGCAGAGTGCTGAAGGTG | GATTCCTGAGAGTCCAAAGACAG |
β-Actin | ATGTGGATCAGCAAGCAGGA | AAGGGTGTAAAACGCAGCTCA |
GAPDH | CATGGCCTTCCGTGTTCCTA | GCGGCACGTCAGATCCA |