Fig. 4

Effect of melatonin pretreatment on myocardial inflammation in septic mice. Representative photographs of immunohistochemical staining of MPO (a), TNF-α (b), IL-6 (c), and NOX2 (d) in mouse heart tissues. e qRT-PCR analysis of TNF-α, IL-6, IL-8, CXCL2, NLRP3, and IL-1β. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus sham or versus CLP; ns, nonsignificant. n = 6 for each group. Statistical analysis of data was performed using ANOVA