Fig. 1

Inhibition of elevated FPR2 is protective against kidney injury. The left renal pedicle of the rat was clamped for 45 min to induce IRI. The right kidney was removed before releasing the clamps. WRW₄ was administered intravenously 30 min before surgery. Serum or kidney tissues were collected 24 h after surgery. A, B Immunofluorescence staining of FPR2 colocalization with CD68⁺ macrophages and MPO⁺ neutrophils. Scale bar, 10 μm. Representative images from one experiment out of three are shown. C Kidney tissues were sectioned for histological examination and TUNEL staining. Scale bar, 100 μm. Representative images from one experiment out of three are shown. D Serum from sham surgery, IRI, and WRW₄-treated rats was sampled at 24 h after surgery. Serum creatinine and blood urea nitrogen levels were measured. E The percentages of FPR2⁺ neutrophils in kidney tissues were determined by flow cytometry. The results are presented as the mean ± SEM from three independent experiments. **P < 0.01, ***P < 0.001