Fig. 3

Knockdown of lncRNA TUG1 inhibits macrophage polarization and inflammation and promotes neuronal axon growth. A After transfection with TUG1 knockdown adenovirus, the protein expression of iNOS was detected by western blotting assay. B After transfection with TUG1 overexpression adenovirus, the protein expression of iNOS was detected by western blotting assay. C After knockdown of TUG1, RT-PCR detected the expression of TNF-α, IL-1α, IL-1β, IL-6, and CXCL2. D After overexpression of TUG1, RT-PCR detected the expression of TNF-α, IL-1α, IL-1β, IL-6, and CXCL2. E, F Immunofluorescence was used to observe the effect of MCM on axonal growth of DRG. Green indicates β-III-tubulin; red indicates NeuN. Scale bar, 50 μm. n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001