Fig. 5

TUG1 and TLR3 are direct targets of miR-1192. A The binding sites of miR-1192 and TUG1 were predicted by starBase database. B The binding sites of miR-1192 and TLR3 were predicted by starBase database. C, D miR-1192-mimics could inhibit the luciferase activity of WT-TUG1 and WT-TLR3, but had no effect on the luciferase activity of MUT-TUG1 and MUT-TLR3. E RT-PCR was used to detect the transfection efficiency of miR-1192 mimics and inhibitors. F, G Western blotting was used to detect the expression level of TLR3 after transfection of miR-1192 mimics and inhibitors. n = 3 per group. *P < 0.05, ***P < 0.001, ****P < 0.0001. ns not significant