Fig. 1
From: Outside the limit: questioning the distance restrictions for cooperative miRNA binding sites
Parameters of cooperative target genes that were selected for the analysis of cooperative miRNA effects by dual luciferase reporter assays. A–E RNA expressional data from a time-course study on the early T cell activation process [15] were utilized for the selection of 15 putative joint targets of miR-21-5p and miR-155-5p to be tested by HiTmIR dual luciferase reporter assays. The hsa-miR-21-5p and hsa-miR-155-5p showed a strong expressional increase upon T cell activation (Donor 1 (A), Donor 2 (B)) and the mRNAs of the selected genes showed expressional decrease during the time-course in both analyzed donors (Donor 1 (C), Donor 2 (D)). Maximum mRNA expression changes compared to the 0 h time-point (log2 fold changes (FCs)) are depicted for the 15 selected genes (E). F The length of distances between in silico predicted binding sites for miR-21-5p and miR-155-5p are given for the 3’UTR sequences of the selected genes. The previously defined distance range for cooperative miRNA binding is highlighted in turquoise color