Fig. 4

Internalization of BILF1 receptors depends on caveolin-1 function. a SNAP-EBV-BILF1 (green), SNAP-PLHV1-BILF1 (blue), SNAP-PLHV2-BILF1 (orange), and empty vector (gray) were expressed in HEK-293A cells together with different concentrations of Cav S80E DNM (black circle 3 ng; black up-pointing triangle 5 ng; black diamond 7 ng). Labeling with the donor SnapLumi4-Tb was performed at 4 °C to prevent any internalization prior to the measurement. Internalization was measured every 4 min for 88 min at 37 °C. The graph curves represent the ratios between donors and acceptors. b The area under the curve (AUC) was calculated for each curve and was normalized to each BILF1 receptor’s AUC in the absence of Cav S80E (100%) and empty vector (0%). Statistical differences were determined by Dunnett’s multiple comparison two-way ANOVA test. **P < 0.01, *** P < 0.001, ****P < 0.0001