Fig. 5
From: Patterns of human and porcine gammaherpesvirus-encoded BILF1 receptor endocytosis
BILF1 receptors interact with Rab7 protein. BILF1 receptors C-terminally tagged with RLuc8 and various concentrations of Rab7-EGFP protein were expressed in HEK-293A cells. BRET1 values are plotted as a function of the ratio between the GFP (total fluorescence) and RLuc8 (total luminescence) signal. Results are fitted using a nonlinear regression equation. BRET50 represents the acceptor/donor ratio at 50% of the BRETmax, indicating the relative affinity of the acceptor (BILF1-RLuc8) for the donor molecules (Rab7-EGFP). Graphs represent the mean (± SEM) from at least three independent experiments