Fig. 4

Intrinsic TGF-β signal modulates myofiber UPR activity under the inflammatory stimulus. A Gene levels of UPR pathway molecules in WT mice injured TA muscle were analyzed by microarray and q-PCR. B, C The protein expression of UPR pathway molecules in WT mice injured TA muscle, or MPC-myotubes receiving pro-inflammatory stimuli or not, analyzed by Western blot. The relative protein level values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total protein]. D Immunofluorescence staining results of ATF6 (white arrow) in damaged TA muscle. All data are presented as means ± SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01). Bar = 50 μm