Fig. 7
Intrinsic TGF-β signaling affects muscle cell immune behaviors by prompting UPR activity under pro-inflammatory conditions. The protein levels of IRE1α and eIF2α (A), H-2Kb, H2-Eα, and TLR3 (B) in control or SM TGF-βr2−/− mice-derived MPC-myotubes, with or without stimulation of IFN-γ/LPS, Tg, or TM, were analyzed by Western blot. The relative protein expression values are expressed as a ratio [protein of interest/GAPDH or phosphorylated (P) protein/total (T) protein]. C mRNA levels of IL-1β, IL-6, and MCP-1 in MPC-myotubes with or without 48 h stimulation of IFN-γ/LPS, Tg, or TM, analyzed by qPCR. All data are presented as means ± SD (n = 3). A one-way ANOVA was used for multiple comparisons (*P < 0.05; **P < 0.01)