Fig. 2

Pre- and postsynaptic activities modulation of PKA catalytic and regulatory subunits protein levels. A Western blot analysis and data quantification of diaphragm samples showing specificity of the anti-Cα, -Cβ, -RIα, -RIβ, -RIIα, and -RIIβ antibodies to show protein bands appear in the correct molecular weight. B Heatmap from proteomic data of relative abundance of PKA subunits in the rat diaphragm samples in basal conditions. C, D Western blot analysis of protein levels after treatment with presynaptic stimulation without contraction—Ctrl versus ES—and with muscle contraction—ES versus ES + C. C Catalytic PKA Cα and Cβ subunits. D Protein kinase A regulatory subunit Iα/Iβ/IIα/IIβ. Data are expressed relative to control (mean ± SD). *p < 0.05, **p < 0.01, and ***p < 0.001 versus the corresponding control. E Pre- and postsynaptic activities modulation of PKA catalytic subunit protein levels (Cβ). Data are expressed relative to control (mean ± SD). *p < 0.05, **p < 0.01, and ***p < 0.001 versus the corresponding control. Prot.Inh., protease inhibitor cocktail. F Effect of presynaptic activity and postsynaptic muscle contraction on the PKA subunits. The diagram is a graphic representation that collectively shows the patterns of changing of the PKA subunits concentrations during presynaptic activity (Ctrl versus ES—blue), contraction abolished by µ-conotoxin GIIIB, and contraction (ES versus ES + C—red). Data are expressed relative to control (black line) (mean). G Graphical representation of the results. Cα/β, protein kinase A catalytic subunit α/β; RIα/RIβ/RIIα/RIIβ, protein kinase A regulatory subunits Iα/Iβ/IIα/IIβ