Fig. 6

Pre- and postsynaptic activities modulation of the PKA targets synthesis and phosphorylation. Western blot analysis and data quantification of the phosphorylated and total levels of A SNAP-25 and B Synapsin-1 in the diaphragm muscle after presynaptic stimulus (Ctrl versus ES) and nerve-induced muscle contraction (ES versus ES + C). Western blot analysis and data quantification of the protein levels and phosphorylation of C SNAP-25 and D Synapsin-1 in the diaphragm muscle after (ES) and (ES + C) with PKA inhibition with H-89. E Graphical representation of the results. Data are expressed relative to control (mean ± SD). *p < 0.05, **p < 0.01, and ***p < 0.001 versus the corresponding control. pSNAP-25, Thr138-phosphorylated synaptosomal-associated protein 25; SNAP-25, synaptosomal-associated protein 25; pSynapsin-1, Ser9-phosphorylated Synapsin-1