Table 1 Antibodies
From: Synaptic retrograde regulation of the PKA-induced SNAP-25 and Synapsin-1 phosphorylation
Target | Epitope | Source | Company (cat. no.) | Dilution |
|---|---|---|---|---|
Cα | Hu Cα C-terminus | Ms mAb | Santa Cruz (sc-28315) | 1/1000 |
Cβ | Hu Cβ C-terminus | Rb pAb | Santa Cruz (sc-904) | 1/1000 |
RIα | Hu RIα residues 1–381 | Ms mAb | Santa Cruz (sc-136231) | 1/1000 |
RIβ | Hu RIβ C-terminus | Ms mAb | Santa Cruz (sc-100414) | 1/1000 |
RIIα | Ms RIIα C-terminus | Rb pAb | Santa Cruz (sc-909) | 1/1000 |
RIIβ | Hu RIIβ residues 21–110 | Ms mAb | Santa Cruz (sc-376778) | 1/800 |
SNAP-25 | Hu SNAP-25 residues around Gln116 | Rb mAb | CST (5309) | 1/1000 |
pSNAP-25 (T138) | Hu SNAP-25 residues around T138 | Rb pAb | Biorbyt (orb163730) | 1/1000 |
Synapsin-1 | Hu Synapsin-1a,b | Rb pAb | AB1543 Chemicon | 1/1000 1/500 |
pSynapsin-1 (S9) | Hu Synapsin-1 residues around S9 | Rb pAb | CST (2311S) | 1/1000 |
AKAP150 | Rat AKAP150 residues 428–449 | Rb pAb | Millipore (07-210) | 1/1000 |
GAPDH | Rb GAPDH | Ms mAb | Santa Cruz (sc-32233) | 1/4000 |
ATPase | Chicken ATPase residues 27–55 | Ms mAb | DSHB (a6f) | 1/2000 |
Syntaxin | Rat Syntaxin | Ms mAb | Millipore (S0664) | 1/1000 |
Secondary antibodies | Anti-Rb conjugated HRP | Dk pAb | 711-035-152 | 1/10000 |
Anti-Ms conjugated HRP | Rb pAb | A9044 | 1/10000 | |
| Â | Anti-Ms conjugated TRITC | Dk pAb | 715-025-151 | 1/1000 |
| Â | Anti-Rb conjugated Alexa fluor 488 | Dk pAb | A21206 | 1/1000 |
|  | α-Bungarotoxin conjugated Alexa Fluor 647 |  | B35450 | 1/1000 |
|  | α-Bungarotoxin conjugated TRITC |  | T1175 | 1/1000 |