Fig. 1

p20BAP31 induced cell apoptosis and inhibited cell proliferation. A Cells were transfected with p20BAP31 for 48 h, and apoptosis was assessed by flow cytometry. B Quantitative analysis of apoptosis rates by flow cytometry. C HCT116 cells were transfected with p20BAP31 for 48 h. Cell nuclei were observed by fluorescence microscopy. Typical apoptosis morphological changes were shown in treated cells, including chromatin condensation and DNA fragmentation. D HCT116 cells were transfected with p20BAP31 for 0, 24, 48, and 72 h. The absorbance value was determined using the CCK-8 assay, the significant difference was compared between the mock group and the p20BAP31 group. E Colony formation was assayed on a 6-well plate for 7 days, and then, the HCT116 cells were fixed with 4% formaldehyde for 30 min and stained with 0.1% crystal violet for 15 min. Representative images, which are shown at × 1 magnification E, and the bar graph of the quantitation F indicate the colony formation of HCT116 cells. The data are shown as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001