Fig. 2

p20BAP31 induced cell cycle arrest at S phase. A Flow cytometry was used to determine the cell cycle distribution of HCT116 cells transfected with p20BAP31 for 48 h. B Statistical analysis of the cell cycle distribution of HCT116 cells. C, D The expression levels of cyclin A2, cyclin E1, and CDK2 in HCT116 cells were measured by western blot analysis after transfection with p20BAP31 for 48 h. *P < 0.05, **P < 0.01, ***P < 0.001