Fig. 3

p20BAP31 reduced MMP with a significant increase in ROS levels in HCT116 cells. A After transfection with p20BAP31 for 48 h, the cells were collected, and DCFH-DA fluorescence was observed by fluorescence microscopy. B Flow cytometry was used to determine the alteration of ROS content in HCT116 cells. C Quantitative analysis of ROS in HCT116 cells. D HCT116 cells were pretreated with 10 μM ML171 and 10 μM Apocynin for 1 h, and then transfected with p20BAP31 for 48 h. DCFH-DA fluorescence was observed by fluorescence microscopy. E Quantitative analysis of the DCFH-DA fluorescence intensity in HCT116 cells. F HCT116 cells were pretreated with 10 μM ML171 and 10 μM apocynin for 1 h and then transfected with p20BAP31 for 48 h. Western blotting was used to detect Bcl-2 and Bax levels. G The relative quantification of the protein levels was analyzed by ImageJ software. H The JC-1 fluorescence distribution in HCT116 cells transfected with p20BAP31 for 48 h was observed under a fluorescence microscope. I Changes in MMP levels in HCT116 cells were detected by flow cytometry analysis. J Quantitative analysis of the mitochondrial membrane potential of HCT116 cells. *P < 0.05, **P < 0.01 and ***P < 0.001