Fig. 4

p20BAP31 induces mitochondrial apoptosis by activating the MAPK signaling pathway. A The expression levels of Bcl-2, Bax, caspase-3, cleaved caspase-3, PARP, cleaved PARP, caspase-9, cleaved caspase-9, and cyt.c proteins in HCT116 cells were determined by western blot after transfection with p20BAP31 for 48 h. B Relative quantity analysis of Bax/Bcl-2 in HCT116 cells. C Western blotting was used to detect p-ERK, ERK, p-JNK, JNK, p-p38, and p38 levels in HCT116 cells transfected with p20BAP31 for 48 h. D The relative quantification of the protein levels was analyzed by ImageJ software. *P < 0.05, **P < 0.01 and ***P < 0.001