Fig. 5

Roles of ROS generation in apoptosis induced by p20BAP31. Cells were transfected with p20BAP31 for 48 h, with or without pretreatment, with 5 mM NAC for 2 h. A Apoptosis of cells was assessed by flow cytometry analyses of annexin V-FITC/PI double-staining after treatment. B Quantitative analysis of apoptosis rates by flow cytometry. C The protein expression levels of Bcl-2, Bax, caspase-3, and cleaved caspase-3 in HCT116 cells were determined by western blotting. D Relative quantities analysis of Bax/Bcl-2 and cleaved caspase-3 in HCT116 cells. E Western blotting was used to detect p-ERK, ERK, p-JNK, JNK, p-p38, and p38 levels in HCT116 cells. F Relative quantities analysis of p-ERK, p-JNK, and p-p38 in HCT116 cells. *P < 0.05, **P < 0.01 and ***P < 0.001