Fig. 6

Roles of JNK in apoptosis induced by p20BAP31. Cells were transfected with p20BAP31 for 48 h, with or without pretreatment, with 10 μM SP600125 for 2 h. A Apoptosis of cells was assessed by flow cytometry analyses of annexin V-FITC/PI double-staining after treatment. B Quantitative analysis of apoptosis rates by flow cytometry. C The protein expression levels of p-JNK, caspase-3, cleaved caspase-3, Bcl-2, and Bax in HCT116 cells were determined by western blotting. D Relative quantities analysis of p-JNK, cleaved caspase-3, and Bax/Bcl-2 in HCT116 cells. **P < 0.01 and ***P < 0.001