Fig. 1

Discovery of DEPs in serum EVs from patients with sepsis-induced lung injury. A PLS-DA of protein profiles of EVs isolated from the serum of patients with sepsis-induced lung injury (n = 3) and healthy control subjects (n = 3). GO analysis of 61 DEPs in the aspects of (B) molecular function, (C) cellular component, and (D) biological process. E KEGG enrichment analysis of the 61 DEPs. F Isolation of serum EVs and ELISA detection of higher DGKK in patients with sepsis-induced lung injury (n = 40) compared with healthy control subjects (n = 20). G Higher endotoxin content in serum of patients with sepsis-induced lung injury (n = 40) compared with healthy control subjects (n = 20). H Pearson correlation analysis showed positive correlation between serum endotoxin content and exosomal DGKK level in patients with sepsis-induced lung injury (n = 40). I ROC curve analysis indicating that exosomal DGKK content could be used to differentiate patients with sepsis-induced lung injury from healthy control subjects. ***P < 0.001 versus control