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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: Extracellular vesicles derived from CD4+ T cells carry DGKK to promote sepsis-induced lung injury by regulating oxidative stress and inflammation

Fig. 3

Toxic effects of CD4+ T-cell-derived EVs on oxidative stress and inflammation on apoptosis, oxidative stress, and inflammation in A549 cells. A549 cells were treated with EVs isolated from CD4+ T cells isolated from healthy subjects treated with (LTE) or without (TE) 10 μg/mL LPS. A Flow cytometric analysis with Annexin V-FITC/PI double staining indicated elevation of cell apoptosis by LTE treatment. Representative plot images from flow cytometry and statistical analysis are shown. B ROS production measured by DCFH-DA staining with flow cytometric analysis showed higher ROS in cells treated with LTE. Representative images from flow cytometry and statistical analysis are shown. C Higher MDA content in cells treated with LTE. D SOD and (E) GPX activities were reduced in cells treated with LTE. Elevated (F) mRNA expression and (G) secretion of TNF-α, IL-1β, and IL-6 in cells treated with LTE. Data presented as mean ± SD. ***P < 0.001 versus control

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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